What is the Colloidal Iron (Muller) Stain Kit?

This stain kit is used for the detection of acid mucopolysaccharides and is based upon the attraction of ferric cations in a colloidal ferric oxide solution for the negatively charged carboxyl and sulfate groups of acid mucins and proteoglycans. The tissue bound ferric ions are visualised by treatment with potassium ferrocyanide to form bright blue deposits of ferric ferrocyanide or prussian blue.

Certified for Diagnostic Use (IVD)

UKAS Compliant: Ensuring your laboratory meets rigorous industry standards.

Pre-Validated and Verified: Consistent performance, helping you save valuable time and resources.

Reliable and Reproducible Results: Backed by proven and documented protocols.

Full Traceability: Manufactured with a robust audit trail, ensuring complete transparency and compliance.

UKCA Marked: Guaranteed regulatory compliance in the UK market.

Full Technical Support: An expert in-house technical team to assist with any queries.

General Information

• Procedure Time: 100 minutes (approximate)
• Shelf Life: 3 Years (from date of manufacture)
• Storage Temperature: Store Perls Reagent 2-8°C. Other components 15-25°C

Kit Contents

• Ferric Chloride 29% Aqueous Solution
• Acetic Acid 25% Aqueous Solution
• Colloidal Perls Reagent A (5% Hydrochloric Acid)
• Colloidal Perls Reagent B (5% Potassium Ferrocyanide)
• Neutral Red 0.5% Aqueous Solution

Results

• Proteoglycans, acid mucins, hyaluronic acid – Bright Blue
• Collagen – Pale Blue
• Nuclei – Red